《植物生理学报》 2018, 54(6): 1137-1143
通信作者：吴卫；E-mail: ewuwei@sicau.edu.cn
摘 要：
为探讨研究红花芽期抗旱性的最佳模拟条件, 并筛选鉴定红花芽期抗旱指标, 以2份红花材料PI305192和PI401472种子为供试材料, 采用6种不同浓度聚乙二醇(PEG-6000)模拟干旱胁迫处理, 运用主成分分析法对多指标予以鉴定筛选。结果表明, 对红花12个指标进行测定分析, 发现浓度20%的PEG-6000可作为研究红花芽期抗旱性的最佳模拟条件, 且在5%~25% PEG处理下, 红花种子发芽率、发芽势、发芽指数、根长、芽长、总长、芽鲜重、根鲜重和总鲜重等均随着胁迫强度的增加呈明显的下降趋势; 而脯氨酸和可溶性蛋白含量随着浓度增加呈增加趋势。主成分分析结果表明, 发芽率、发芽势、丙二醛含量、总长和总鲜重等5个指标可作为红花芽期抗旱鉴定筛选的主要指标。关键词：红花; 干旱胁迫; 种子萌发; 鉴定指标; 主成分分析
收稿：2017-10-25 修定：2018-05-09
资助：国家自然科学基金(81274020)。
Corresponding author: WU Wei; E-mail: ewuwei@sicau.edu.cn
Abstract:
To study the optimum conditions of simulated drought stress and screen the indexes of drought resistance at germinating stage, the seeds of safflower (Carthamus tinctorius) PI305192 and PI401472 were used as the tested materials under the simulated drought conditions with six different concentrations of PEG-6000, and the principal component analysis method was used to identify and screen multiple indicators. The results showed that 20% PEG-6000 could be the optimum condition for the simulation of drought stress. The germination rate, germination potential, germination index, root length, bud length, total length, germination fresh weight, root fresh weight and total fresh weight decreased with the increase of stress intensity under the treatments of 5%–25% PEG, whereas the contents of proline and soluble protein increased. The principal component analysis results showed that the five indexes, including germination rate, germination potential, malondialdehyde, total length and total fresh weight, could be selected as the main indexes for drought resistance identification of safflower at germinating stage.Key words: safflower (Carthamus tinctorius); drought stress; seed germination; principal component analysis; identification index
此摘要已有 1338 人浏览
